Oxidation State Distributions Provide Insight into Parameters Directing the Assembly of Metal–Organic Nanocapsules

Human Studies Provide Insight into the Pathogenesis

Sir—In their article about Cryptosporidium species, Leav et al. [1] provide a nice summary of data on the molecular biology of these parasites and findings from animal studies. However, there are also recent human studies on the pathogenesis, immunology, and treatment of infection with this organism that were not discussed in the article. For example, the section on pathogenesis states that the...

Structures of PGAM5 Provide Insight into Active Site Plasticity and Multimeric Assembly

PGAM5 is a mitochondrial membrane protein that functions as an atypical Ser/Thr phosphatase and is a regulator of oxidative stress response, necroptosis, and autophagy. Here we present several crystal structures of PGAM5 including the activating N-terminal regulatory sequences, providing a model for structural plasticity, dimerization of the catalytic domain, and the assembly into an enzymatica...

De Novo Assembly and Comparative Transcriptome Analysis Provide Insight into Lysine Biosynthesis in Toona sinensis Roem

Toona sinensis Roem is a popular leafy vegetable in Chinese cuisine and is also used as a traditional Chinese medicine. In this study, leaf samples were collected from the same plant on two development stages and then used for high-throughput Illumina RNA-sequencing (RNA-Seq). 125,884 transcripts and 54,628 unigenes were obtained through de novo assembly. A total of 25,570 could be annotated wi...

Mitophagy: New Insight into the Cardiovascular Aging  

Structural insight into TPX2-stimulated microtubule assembly

During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The molecular mechanism of how TPX2 stimulates MT assembly remains unknown because structural information about the interaction of TPX2 with MTs is lacking. Here, we determine the cryo-electron microsco...